Cell Banking
A cell bank provides a stable uniform source of hybridoma cells for
monoclonal antibody production. Your cell line is used for generation
of a Platinum Master Cell Bank or a Gold Master Cell Bank and a
Manufacturer’s Working Cell Bank. Before freezing the cell bank, the
clonality of the cell line is determined and the isotype and isoelectric
point of the monoclonal antibody is determined. After the cell bank is
frozen, cryovials are thawed and the same tests are performed on the
cells in the post-freeze culture and the monoclonal antibody. When post
freeze is completed, the cell bank undergoes mycoplasma and sterility
testing.
Platinum Master Cell Banks
Viability tested at 0, 24 and 48 hours. Isotype confirmed.
Samples sent for mycoplasma testing.
Limited dilution cloning is performed and four highest producing clones are selected by isotype-specific ELISA.
The four highest producers are expanded and antibody concentration is tested by redial immunodifusion assay.
The clone with the highest antibody concentration is selected and an additional limited dilution cloning is performed to confirm clonality or greater than 85% prior to freezing the bank.
Freeze 52 vials
Perform post thaw testing; viability, isotype and limited dilution cloning on frozen cells. Verify antibody banding properties by isoelectric focusing.
Samples sent for
Sterility Testing
Mycoplasma Testing
MAP Biological Testing for following: Sendai virus, Mouse hepatits virus, Pneumonia virus, minute virus, Mouse parvovirus (1,2 and 3), Theller’s murine encephalomyelitis virus, Lymphocytic chorlomeningtis virus, Polyoma virus, Lactate cytomegalovirus, K virus, Mouse thymic virus and Hantaan virus.
Master Cell Bank Report
Includes one year free, dual site storage at Newark, DE and Windham, ME locations.
Gold Master Cell Bank
Viability tested at 0, 24 and 48 hours. Isotype confirmed.
Samples sent for mycoplasma testing.
Limited dilution cloning is performed and four highest producing clones are selected by isotype-specific ELISA.
The four highest producers are expanded and antibody concentration is tested by redial immunodifusion assay.
The clone with the highest antibody concentration is selected and an additional limited dilution cloning is performed to confirm clonality or greater than 85% prior to freezing the bank.
Freeze 52 vials
Perform post thaw with 1 vial testing; viability, isotype and limited dilution cloning on frozen cells. Verify antibody banding properties by isoelectric focusing.
One Vial sent for
Sterility Testing
Mycoplasma Testing
Common Murine Viral Testing for following: Sendai virus, Mouse hepatits virus, Pneumonia virus, minute virus, Mouse parvovirus (1,2 and 3), Theller’s murine encephalomyelitis virus
Master Cell Bank Report
Includes one year free, dual site storage at Newark, DE and Windham, ME locations.
Manufacturer’s Working Cell Bank
Starting vial must be from an SDIX generated Master Cell Bank
Viability tested at 0, 24 and 48 hours. Isotype confirmed.
Limited dilution cloning is performed to confirm > 85% clonality prior to freezing the bank.
Freeze 25 vials
Perform post thaw with 1 vial testing; viability, isotype and limited dilution cloning on frozen cells. Verify antibody banding properties by isoelectric focusing.
Manufacturer’s Working Cell Bank Report is generated.
Includes one year free storage at the Newark, DE site
Research Grade Bank
Viability tested at 0, 24 and 48 hours. Isotype confirmed.
Samples sent for mycoplasma testing.
Freeze 21 vials
One Vial sent for
Mycoplasma Testing
Common Murine Viral Testing for following: Sendai virus, Mouse hepatits virus, Pneumonia virus, minute virus, Mouse parvovirus (1,2 and 3), Theller’s murine encephalomyelitis virus
Certificate of Analysis for bank
Includes one year free storage at the Newark, DE site.