Species Protocols

SDIX offers a variety of species for antibody development. Choosing which species to use could have an impact on if the antibody is successful.  Our scientists work with you to ensure all of the right questions and decisions are made for the best chance of success.

In addition to the species listed below we also offer standard protocols for mice, rats, hamsters and guinea pigs upon request.

You may send in your antigen, peptide or protein, to us and we take it from there. The protocol that you have chosen will begin within 5 business days of receipt of the antigen and project submission form.  You may choose the SDIX Standard Protocols, which have an extensive history of success, or utilize your own protocol, whichever meets your needs.

Rabbit – 70 Day Standard 2 Rabbit Protocol

All rabbits are Specific Pathogen Free (SPF). Healthy animals make great antibodies.
Mean serum yields 50-75 mL of antiserum per rabbit.
Mean IgG concentration in immune rabbit sera is 4 mg/mL.
Mean specific antibody concentration is 0.15 to 0.5 mg/mL.
Antigen requirement: 1.6 – 2.0 mgs per 2-rabbit protocol

 

DAY ACTIVITY
0 Pre-immune sera collection
0 Injection
21 Injection
35 Injection
44-45 Sera collection
49 Injection
58-59 Sera collection
63 Sera collection
66 Ship sera
70 Extend or end project

Goat / Sheep 200 Day Standard Protocol

All animals are Blue Tongue Disease Free. Healthy animals make great antibodies.
No Blue Tongue means our plasma is easily exported to other countries.
Yield is 1,100 ml defibrinated plasma.
Antigen requirement: 2.4 to 5.0 mgs.

 

DAY ACTIVITY
0 Pre-immune sera collection
0 Injection
14 Injection
28 Injection
42 Sera collection
56 Sera collection
56 Injection
Every 14 days thereafter Injection
Every 28 days thereafter Sera collection
168 Sera collection
168 Injection
177 Plasmapheresis
179 Plasmapheresis
200 Extend or end project

 

If expected titer is not obtained at day 168, the project can be extended and plasmapheresis is not performed until expected titers are reached.

Chicken 59 Day (yolks) Standard 2 Chicken Protocol

Yield is 6-12 eggs per chicken. 3 eggs per chicken guaranteed.
IgY yield is approximately 50 mgs per yolk.
Antigen requirement: 1.6 to 3.0 mgs per 2-chicken project.

 

DAY

ACTIVITY

-4 Pre-immune egg collection
-3 Pre-immune egg collection
-2 Pre-immune egg collection
-1 Pre-immune egg collection
0 Injection
14 Injection
28 Injection
42 Injection
42-56 Egg collection
59  Ship

Chicken 79 Day (Purified IgY) Standard 2 Chicken Protocol

Yield is 6-12 eggs per chicken. 3 eggs per chicken guaranteed).
IgY yield is approximately 50 mgs per egg.
Antigen requirement: 1.6 to 3.0 mgs per 2-chicken project.

 

DAY ACTIVITY
-4 Pre-immune egg collection
-3 Pre-immune egg collection
-2 Pre-immune egg collection
-1 Pre-immune egg collection
0 Injection
14 Injection
28 Injection
42 Injection
42-56 Egg collection
58 IgY purification
79 Ship

Element

Become a Member


Continue