SDIX offers a variety of species for antibody development. Choosing which species to use could have an impact on if the antibody is successful. Our scientists work with you to ensure all of the right questions and decisions are made for the best chance of success.
In addition to the species listed below we also offer standard protocols for mice, rats, hamsters and guinea pigs upon request.
You may send in your antigen, peptide or protein, to us and we take it from there. The protocol that you have chosen will begin within 5 business days of receipt of the antigen and project submission form. You may choose the SDIX Standard Protocols, which have an extensive history of success, or utilize your own protocol, whichever meets your needs.
Rabbit – 70 Day Standard 2 Rabbit Protocol
All rabbits are Specific Pathogen Free (SPF). Healthy animals make great antibodies.
Mean serum yields 50-75 mL of antiserum per rabbit.
Mean IgG concentration in immune rabbit sera is 4 mg/mL.
Mean specific antibody concentration is 0.15 to 0.5 mg/mL.
Antigen requirement: 1.6 – 2.0 mgs per 2-rabbit protocol
| DAY | ACTIVITY |
| 0 | Pre-immune sera collection |
| 0 | Injection |
| 21 | Injection |
| 35 | Injection |
| 44-45 | Sera collection |
| 49 | Injection |
| 58-59 | Sera collection |
| 63 | Sera collection |
| 66 | Ship sera |
| 70 | Extend or end project |
Goat / Sheep 200 Day Standard Protocol
All animals are Blue Tongue Disease Free. Healthy animals make great antibodies.
No Blue Tongue means our plasma is easily exported to other countries.
Yield is 1,100 ml defibrinated plasma.
Antigen requirement: 6.5 to 13 mgs.
| DAY | ACTIVITY |
| 0 | Pre-immune sera collection |
| 0 | Injection |
| 14 | Injection |
| 28 | Injection |
| 42 | Sera collection |
| 56 | Sera collection |
| 56 | Injection |
| Every 14 days thereafter | Injection |
| Every 28 days thereafter | Sera collection |
| 168 | Sera collection |
| 168 | Injection |
| 177 | Plasmapheresis |
| 179 | Plasmapheresis |
| 200 | Extend or end project |
If expected titer is not obtained at day 168, the project can be extended and plasmapheresis is not performed until expected titers are reached.
Chicken 59 Day (yolks) Standard 2 Chicken Protocol
Yield is 6-12 eggs per chicken. 3 eggs per chicken guaranteed.
IgY yield is approximately 50 mgs per yolk.
Antigen requirement: 1.6 to 3.0 mgs per 2-chicken project.
|
DAY |
ACTIVITY |
| -4 | Pre-immune egg collection |
| -3 | Pre-immune egg collection |
| -2 | Pre-immune egg collection |
| -1 | Pre-immune egg collection |
| 0 | Injection |
| 14 | Injection |
| 28 | Injection |
| 42 | Injection |
| 42-56 | Egg collection |
| 59 | Ship |
Chicken 79 Day (Purified IgY) Standard 2 Chicken Protocol
Yield is 6-12 eggs per chicken. 3 eggs per chicken guaranteed).
IgY yield is approximately 50 mgs per egg.
Antigen requirement: 1.6 to 3.0 mgs per 2-chicken project.
| DAY | ACTIVITY |
| -4 | Pre-immune egg collection |
| -3 | Pre-immune egg collection |
| -2 | Pre-immune egg collection |
| -1 | Pre-immune egg collection |
| 0 | Injection |
| 14 | Injection |
| 28 | Injection |
| 42 | Injection |
| 42-56 | Egg collection |
| 58 | IgY purification |
| 79 | Ship |
