SDIX Genomic Antibody Technology™ is a highly optimized form of DNA immunization. The successful application of DNA immunization requires the optimization of many diverse steps in order to consistently produce high titers. SDIX has systematically optimized DNA immunization to the point that it now provides many benefits, including:
• Elimination of the need for users to supply antigen protein or peptide material
• Lack of purification steps bypasses the issues caused by contamination of proteins or peptides
• In vivo expression of the antigen improves the probability of exposing native epitopes to the immune system
Because it lacks the upfront need for proteins or peptides, GAT is a simplified process that typically delivers antibodies in a shortened time frame. The process occurs as follows:
• User submits protein sequence (or DNA code) as electronic information
• Bioinformatic analysis is used to determine best possible protein antigen sequence
• Plasmid encoding the antigen sequence is introduced into the rabbit via transfection
• Expression of the protein in vivo occurs
• Protein is recognized by immune system of host rabbit
Use of Rabbits as Hosts for SDIX Genomic Antibodies™
The use of rabbits as host organisms for SDIX Genomic Antibodies maintains the benefits listed above while improving antibody yields and minimizing the costs associated with increasing production scale. Furthermore, experimental evidence shows that rGAT antibodies also outperform conventional antibodies across the spectrum of downstream uses, including Western blotting, ELISA assays, and Immunohistochemistry (IHC).
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The sensitivity of SDIX Genomic Antibodies in sandwich ELISA assays was compared to the sensitivity
of antibodies generated by peptide immunizations. In each set of assays, the SDIX Genomic Antibody
and the conventional antibody were paired with the identical second antibody.
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The performance
of SDIX Genomic Antibodies in western blot assays was benchmarked against the
performance of antibodies generated by peptide immunizations. Western blots were scored using a modified
HPR rating system, with 1 being best and 5 being worst (proteinatlas.org).
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Extension steps added to SDIX Genomic Antibody Process
To further enhance the flexibility of the SDIX Genomic Antibody offering, we have introduced extension steps into the process. The availability of extensions allow end users to:
• Ensure access to future quantities of an antibody without large upfront costs
• Screen antibodies for desired characteristics prior to large-scale production
• Dramatically scale up production of antibodies from chosen host animals
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The
yields of SDIX Genomic Antibodies are shown following the standard production
process, following a single extension, and following three extensions.
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The average titer of antibody for six different Genomic Antibody projects is shown for ten consecutive extensions.
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Timelines for SDIX Genomic Antibody Production