RapidChek® CONFIRM™ non-O157 STEC Immunomagnetic Separation (IMS) Kit
RapidChek CONFIRM non-O157 STEC Immunomagnetic Separation (IMS) Kit
The RapidChek® CONFIRM™ non-O157 STEC IMS Kit
is designed for the confirmation of the presence of the “Big Six” non-O157 STECs – O26, O45, O103, O111, O121, O145 - in beef samples. The RapidChek CONFIRM IMS Kit isolates E. coli O26, O45, O103, O111, O121 and O145 in the enriched potential positive samples for confirmation on conventional, selective, confirmation agars. By isolating the non-O157 STECs, the use of this confirmation kit ultimately increases the accuracy and repeatability and reduces the labor and time required for confirmation.
The RapidChek and RapidChek CONFIRM line of pathogen detection tools are designed to provide a practical, real-world solution that does not require capital expense or extensive training. For 20 years, SDIX
has created application-driven solutions that protect your customers and your business. Our integrated pathogen detection solutions rely on our 20 years of expertise and experience in protein detection.
Benefits of RapidChek CONFIRM non-O157 STEC
• Isolate E. coli O26, O45, O103, O111, O121 and O145 present within an enriched sample
• Increases the chances of identifying a “Big Six” non-O157 STEC from selective agar plates
• Easy to use technology requiring no additional instrumentation
How Does RapidChek CONFIRM Work?
This confirmation kit uses specific, high-affinity antibodies each attached to separate magnetic particles for isolation of E. coli O26, O45, O103, O111, O121 and O145 from enriched samples.
For confirmation, each coated magnetic particle solution is added to the potential positive enrichment and incubated. If E. coli O26, O45, O103, O111, O121 or O145 are present, it will bind to the
magnetic particles via the antibody. A magnet is then used to concentrate the bound magnetic particles and the remaining enrichment is discarded leaving only magnetic particles bound to the E. coli O26, O45, O103, O111, O121 or O145. Confirmation procedures are then continued as per the reference method with the concentrated sample.
Figure 1. How RapidChek CONFIRM Works: Example O26 isolation.
Why is RapidChek CONFIRM needed to confirm potential positive samples?
Within an enriched meat sample, there can be mixed populations of E. coli and other organisms typically present, creating a challenging environment for isolation of “Big Six” O-type isolates. The use of the RapidChek CONFIRM IMS kit reduces the presence of other non-O157 E. coli species by concentrating the “Big Six” O-types and washing away irrelevant bacteria. When the sample is plated to selective agar for confirmation, E. coli O26, O45, O103, O111, O121 and O145 are the predominant colonies that form increasing the likelihood of picking that organism if present within the sample. By reducing the presence of other non-O157 E. coli species, the accuracy and repeatability of the cultural confirmation is increased and the labor and time is decreased.
Validated Applications
The RapidChek CONFIRM non-O157 STEC IMS Kit has been listed by the USDA FSIS in MLG 5B.02 for use in the confirmation of E. coli O26, O45, O103, O111, O121 or O145 potential positive beef samples.