Two
Peptide Antibody
(Peptides are designed for different regions of the same protein)
1. Peptide design (against 2 different epitopes of the same protein)
Consideration for -
- Antigenicity, N terminal and C terminal options
- Solubility (hydrophilicity & hydrophobicity)
- Structural concerns, interactions and presence of a.acid residues
- Specificity/cross reactivity – paralogs, orthologs
- Bioinformatics
- Jameson & Wolf combination surface probability(1988)
- Homology (Blast Analysis)
Recommendation for multiple peptides
Selection of 2 peptides based on assay needs (free consultation with SDIX Scientists)
2. Peptide synthesis and preparation
- >90% pure, 5mgs of each peptide
- Conjugation of peptides
3. Antibody development (Polyclonal)
- Number of rabbits: 4 (2 for each peptide)
- Protocol - 4 injections/3 bleeds
- ELISA - 2 plate
4. Deliverables:
- Project schedule
- Pre-bleed & 2 ml samples from each production bleed (12)
- 90 – 132mls serum per peptide
- ELISA report
- Peptide balance with project term
Total project turnaround – 78 business days, 109 calendar days