Phospho-Peptide
Antibody Package (Affinity Purified)
1. Peptide design
Consideration for -
- Antigenicity, N terminal and C terminal options
- Solubility (hydrophilicity & hydrophobicity)
- Structural concerns, interactions and presence of a.acid residues
- Specificity/cross reactivity – paralogs, orthologs
- Bioinformatics
- Jameson & Wolf combination surface probability(1988)
- Homology (Blast Analysis)
Recommendation for multiple peptides
Selection of peptide (based on assay needs -consultation with customers)
2. Peptide synthesis and preparation
- >90% pure, 35mgs of peptide
- Conjugation of peptide
3. Antibody development (Polyclonal)
- Number of rabbits: 2
- Protocol - 5 injections/5 bleeds
- ELISA - 2 antigens, 4 total plates
- Affinity Purification
4. Deliverables:
- Project schedule
- 1.5 – 9.0 mgs purified antibody
- Sterile filtered
-
Columns (2)
- Final project report
- Peptide balance with project term
Total project turnaround – 98 business days, 137 calendar days