Affinity Purified
Single Peptide Antibody Package
1. Peptide
design
Consideration for -
- Antigenicity,
N terminal and C terminal options
- Solubility (hydrophilicity
& hydrophobicity)
- Structural concerns, interactions
and presence of a.acid residues
- Specificity/cross reactivity
– paralogs, orthologs
- Bioinformatics
- Jameson & Wolf combination
surface probability(1988)
- Homology (Blast
Analysis)
Recommendation for multiple peptides
Selection of peptide (based on assay needs -consultation with customers)
2. Peptide
synthesis and preparation
- >90%
pure, 20 mgs of peptide
- Conjugation
of peptide
3. Antibody
development (Polyclonal)
- Number
of
rabbits: 2
- Protocol
- 4 injections/3 bleeds
- Affinity
Purification - Large, 1 pass
- ELISA
-
1 plate
4. Deliverables:
- Project schedule
- 1.5 – 9.0 mgs purified
antibody
- Sterile filtered
- SDS page gel
- Flow Through
- Columns
- Final project report
- Peptide balance with project term
Total project turnaround - 88 business days, 123 calendar days