Alpha-1 Antitrypsin (AAT) is a 394aa monomeric, glycosylated protein found in serum. Scientists at SDIX designed 10 DNA antigen sequences within AAT by “walking” down the protein and using each of the sequences as a plasmid insert for SDIX’s Genomic Antibody Technology™.
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The AAT sequence was systematically broken up into a series of 10 overlapping regions and each construct was immunized into a group of 3 mice using Genomic Antibody Technology™. The antisera were tested
by western blot (recombinant protein fragment) and ELISA (native protein). Models of the selected domains are highlighted in yellow.
