In Vivo Production
Monoclonal antibody is produced in vivo in ascites fluid. Production in ascites fluid is nearly always the most economical method of monoclonal antibody production; your cost per milligram of monoclonal antibody is the lowest. Monoclonal antibody produced in ascites fluid is typically purified before shipment. For monoclonal antibodies produced repeatedly in large quantities, SDIX offers an ascites optimization process to maximize production efficiency and minimize animal use.
All incoming cell lines require pre-production verification.
Includes:
- Viability check of cell line
- Isotype Testing
- Strains: Balb/c, ICR SCID, CAF1 are available, please contact us for additional strains
- Lot Size 10 -2000 mice per lot (> 500 mice needed, please call for availability)
- Ascites fluid is harvested over multiple taps
- At completion all taps are pulled and centrifuged
- Final IgG concentration is determined
- Final product stored at -20oC
- Average ascites volume per mouse is 3-5mls/mouse and average antibody concentration is 3-5mg/mL.
- Timing 2-3 months
Optional Services
- Limited Dilution Cloning (Verify monoclonality of cell line)
- Mycoplasma testing
- Murine pathogen testing Mouse parvo virus
- Mouse hepatits virus
- Pneumonia virus
- Sendai virus
- Minute virus
In Vivo Purification Services
Platinum Package
- Perform Protein A or Protein G Purification
- Full lot records documentation
- Custom Final Buffer Formulation
- Custom Final Concentration
- Custom Packaging (up to 25 vials)
- Final QC
- Isotype
- HPLC Purity
- Immunoelectrofocusing gel
- Certificate of Analysis
- Timing – 3 weeks
Gold Package
- Documentation in form of data sheet
- Perform Protein A of Protein G Purification
- Concentration >1mg/mL
- Buffer Formulation is PBS or PBSA
- Bulk Packaging
- Final QC
- Certificate of Analysis
- Timing – 3 weeks