Let us design your peptides. We have qualified scientists that will collaborate with you to determine the best peptide design for the antibody that you need. These peptide packages start from the design and finish with either serum or affinity purified antibody depending on your needs. We also can design dual peptide projects to enhance your chances of success.
Consideration for -
-
Antigenicity, N terminal and C terminal options
- Solubility (hydrophilicity & hydrophobicity)
- Structural concerns, interactions and presence of a.acid residues
- Specificity/cross reactivity – paralogs, orthologs
- Bioinformatics
- Jameson & Wolf combination surface probability(1988)
- Homology (Blast Analysis)
- Selection of peptide (based on assay needs -consultation with customers)
Two Peptide Antibody Package (Serum)
(Peptides are designed for different regions of the same protein)
Total project turnaround – 78 business days, 109 calendar days
- Peptide design (against 2 different epitopes of the same protein)
- Peptide synthesis and preparation
>90% pure, 5mgs of each peptide
Conjugation of peptides
- Antibody development (Polyclonal)
Number of rabbits: 4 (2 for each peptide)
Protocol - 4 injections/3 bleeds
ELISA - 2 plate
- Deliverables:
-
Project schedule
- Pre-bleed & 2 ml samples from each production bleed (12)
- 90 – 132mls serum per peptide
-
ELISA report
-
Peptide balance with project term
Two Peptide Antibody Package (Affinity Purification)
(Peptides are designed for different regions of the same protein)
Total project turnaround – 88 business days, 123 calendar days
- Peptide design (against 2 different epitopes of the same protein)
- Peptide synthesis and preparation
>90% pure, 10mgs of each peptide
Conjugation of peptides
- Antibody development (Polyclonal)
Number of rabbits: 4 (2 for each peptide)
Protocol - 4 injections/3 bleeds
Affinity Purification - Large, 1 pass
ELISA - 2 plates
- Deliverables:
- Project schedule
- 1.5 – 9.0 mgs purified antibody per peptide
- Sterile filtered
-
Flow Through - optional
- Columns
-
Final project report
- Peptide balance with project term
Single Peptide Antibody Package
Total project turnaround – 78 business days, 109 calendar days
- Peptide design
- Peptide synthesis and preparation
>90% pure, 5mgs of peptide
Conjugation of peptide
- Antibody development (Polyclonal)
Number of rabbits: 2
Protocol - 4 injections/3 bleeds
ELISA - 1 plate
- Deliverables:
- Project schedule
- Pre-bleed & 2 ml samples from each production bleed (6)
- 90 – 132mls serum per peptide
- ELISA report
- Peptide balance with project term
Affinity Purified Single Peptide Antibody Package
Total project turnaround – 88 business days, 123 calendar days
- Peptide design
- Peptide synthesis and preparation
>90% pure, 20 mgs of peptide
Conjugation of peptide
- Antibody development (Polyclonal)
Number of rabbits: 2
Protocol - 4 injections/3 bleeds
Affinity Purification - Large, 1 pass
ELISA - 1 plate
- Deliverables:
- Project schedule
- 1.5 – 9.0 mgs purified antibody
- Sterile filtered
- Flow Through - optional
- Columns
- Final project report
- Peptide balance with project term
Phospho-Peptide Antibody package (Affinity Purified)
Total project turnaround – 98 business days, 137 calendar days
- Peptide design
- Peptide synthesis and preparation
>90% pure, 53 mgs of peptide
Conjugation of peptide
- Antibody development (Polyclonal)
Number of rabbits: 2
Protocol - 5 injections/5 bleeds
ELISA - 2 antigens, 4 total plates
Affinity Purification
- Deliverables:
- Project schedule
- 1.5 – 9.0 mgs purified antibody
- Sterile filtered
-
Flow Through (1)-optional
- Columns (2)
- Final project report
- Peptide balance with project term
Next Steps
Do you have a question about your polyclonal antibody? Let our team of experts help
you.
Are you ready to get started
with your polyclonal project? Submit the Project
Submission form.